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Wednesday, August 12, 2020 | History

3 edition of Isolation, purification, and characterization of a Shigella Phage found in the catalog.

Isolation, purification, and characterization of a Shigella Phage

M. I. Huq

Isolation, purification, and characterization of a Shigella Phage

by M. I. Huq

  • 314 Want to read
  • 7 Currently reading

Published by International Centre for Diarrhoeal Disease Research, Bangladesh in Dacca .
Written in English

    Places:
  • Bangladesh.
    • Subjects:
    • Shigellosis -- Bangladesh.

    • Edition Notes

      StatementM.I. Huq, M.A. Salek.
      SeriesScientific report / International Centre for Diarrhoeal Disease Research, Bangladesh ;, no. 27
      Classifications
      LC ClassificationsMLCM 93/06261 (R)
      The Physical Object
      Pagination13 leaves ;
      Number of Pages13
      ID Numbers
      Open LibraryOL4249216M
      LC Control Number80908196

      Isolation and characterization of antibodies against three consecutive Tn-antigen clusters from a phage library displaying human single-chain variable fragments. Journal of Biochemistry , (6), DOI: /jb/mvq We review some aspects of the rapid isolation of, screening for and characterization of jumbo phages, i.e., phages that have dsDNA genomes longer than Kb. The first aspect is that, as plaque-supporting gels become more concentrated, jumbo phage plaques become smaller. Dilute agarose gels are better than conventional agar gels for supporting plaques of both jumbo phages and, prospectively.

      Isolation and characterization of phage. In previous study, Jun and colleagues reported two virulent Shigella phages, a Siphoviridae phage pSf-1 infecting S. flexneri 11 and a Podoviridae phage pSb-1 infecting S. boydii pSf-1 was able to infect all of the S. flexneri and most of S. sonnei strains, forming clear plaques 11; pSb-1 was able to infect all of the S. boydii strains and formed. A bacteriophage encoding the Shiga toxin 2c variant (Stx2c) was isolated from the human Escherichia coli O strain CB and shown to form lysogens on the E. coli K laboratory strains C and MG Production of Stx2c was found in the wild-type E. coli O strain and the K lysogens and was inducible by growing bacteria in the presence of ciprofloxacin. Phage is the first.

      Phage DNA Isolation Kit This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. During recent years, interest in the use of bacteriophages as biocontrol agents against foodborne pathogens has increased, particularly for members of the family Enterobacteriaceae, with pathogenic Escherichia coli, Shigella, and Salmonella strains among them. Here, we report the isolation and characterisation of 12 novel T5-like bacteriophages from confiscated food samples.


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Isolation, purification, and characterization of a Shigella Phage by M. I. Huq Download PDF EPUB FB2

Isolation, Characterization and Genomic Analysis of a Novel Lytic Bacteriophage vB_SsoS-ISF Infecting Shigella Sonnei and Shigella Flexneri. These results indicated that vB_SsoS-ISF is a novel virulent T1virus phage and may have potential as an alternative treatment for shigellosis.

These results indicated that vB_SsoS-ISF is a novel virulent T1virus phage and may have potential as Cited by: 6. Isolation, characterization and genomic analysis of a novel lytic bacteriophage vB_SsoS-ISF infecting Shigella sonnei and Shigella flexneri.

Shahin K(1)(2), Bouzari M(1), Wang R(2). Author information: (1)Department of Biology, Faculty of Sciences, University of Isfahan, Hezar Jereeb Street,Isfahan, by: 6. Isolation and purification of phage was then performed using the double-layer agar technique. Briefly, the filtrate was serially diluted in saline magnesium (SM) buffer ( M Tris-HCl buffer, pHcontaining M NaCl, M MgSO 4, and % gelatin), mixed with µL of overnight host culture and 4 mL of molten top agar [LB broth Cited by: 1.

Isolation, characterization, and PCR-based molecular identification of a siphoviridae phage infecting Shigella dysenteriae Author links open overlay panel Khashayar Shahin a Hongduo Bao a Majid Komijani b Mohadeseh Barazandeh a Majid Bouzari c Abolghasem Hedayatkhah d e Lili Zhang a Hang Zhao a Tao He a Maoda Pang a Ran Wang a fCited by: 2.

The first report on the Shigella isolation and characterization was published by Kiyoshi Shiga in [8]. The genus Shigella is classified in the family Enterobacteriaceae. It is gram negative bacilli, non-spore forming, non-motile, µm in size and facultative anaerobic pathogen that.

The phage lysates (4 μL) at a concentration of 10 8 PFU/mL were spotted onto the lawns of the bacterial strains, Isolation incubated at 37 °C for 18–24 h. The host range and characterization of a Shigella Phage book the phages was assessed on each test strain by a common scoring system (Clokie & Kropinski, a).

Characterization of phage LPST10 Growth curve of phage LPST The overall purpose of the PHAGES lab was to capture (get the phage from the environment), tame (isolate/purify a single phage), dissect (analyze the phage’s physical and molecular structure), analyze (elucidate the genomic properties), discover (identify novel genomic traits), and share (disseminate data) their individual phages.

Phage morphologies and genome sizes 9 Replication cycles of lytic and lysogenic bacteriophages 11 Resistance pattern in Shigella flexneri from to 20 Incidence of different subtypes of Shigella from to 21 in (A) Europe-America and (B) Asia-Africa. Phage titre determination Investigation of phage susceptibility on a variety of host range Plaque purification Characterization: 1) Molecular characterization of nucleic acid: Restriction enzymes/PCR/PAGE 2) Bacteriophagemorphology-TEM study Phage propagation in bulk and preservation Isolation of different bacteria Bacterial identification.

ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGE FROM RAW SEWAGE SPECIFIC FOR Escherichia coli OH7 by SITI FARIZA BT JUHARUL ZAMAN Thesis submitted in.

Shigella flexneri is the major cause of shigellosis in the developing countries. The O-antigen component of the lipopolysaccharide is one of the key virulence determinants required for the pathogenesis of S. flexneri. The glucosyltransferase and/or acetyltransferase genes responsible for the modification of the O-antigen are encoded by temperate serotype converting bacteriophage present in.

Phage suspension containing (1 × 10 7 pfu) was incubated at different temperatures of 30, 40, 60, 55, 70, 80, 90, and °C for 10 min. Phage infectivity was assayed by the spot test and the double over layer agar technique. Isolation and characterization of nucleic acids from phage.

Lytic phage isolation and purification. In order to isolate the lytic bacteriophage against S. dysenteriae (Persian Type Culture Collection ; PTCC ), the conventional phage isolation protocol was used with slight modifications.

Several samples of untreated wastewater and sewage from urban, hospital and livestock (Jiangsu province. Isolation, characterization, and PCR-based molecular identification of a siphoviridae phage infecting Shigella dysenteriae.

Phage morphological and genetic characteristics were studied using TEM, and sequencing, respectively. In addition, the genome size was estimated, and phage resistance to different temperatures and pH, host range.

The genome sequence, morphology, and genetic features of a novel phage, named SSE1, is reported here. Phage SSE1 that infects Shigella dysenteriae (China General Microbiological Culture Collection Center number: ) was isolated from the aeration tank water of a sewage treatment plant.

SSE1 showed morphological features associated with those of phages in Myoviridae. Figure 3: Plaque Purification- Successive rounds of picking isolated plaques, making dilutions, infecting M.

smegmatis, and plating allow for the purification and isolation of a single phage. The plaque purification steps must be repeated until a purified phage is obtained, reflected by a single plaque morphology.

Two examples are shown above. The isolation of bacteriophages for phage therapy is often presented as a fairly straightforward exercise of mixing a phage-containing sample with host bacteria, followed by a simple removal of bacterial debris by filtration and/or centrifugation the next day [1,2,3].Indeed, when questions of bacterial resistance to therapeutic phages are discussed, it is common to hear some variation of “in.

Shigellosis, caused by Shigella, is a major global health concern, with nearly million cases and over a million deaths occurring annually worldwide.

Shigella flexneri is one of the most common subgroups of Shigella with a high incidence of multidrug-resistance.

The phage therapy approach is an effective method for controlling multidrug-resistant bacteria. However, only a few Shigella. Isolation & Identification of Shigella species from food and water samples of Quetta, Pakistan Article (PDF Available) February with 2, Reads How we measure 'reads'.

Abstract. Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C.

jejuni lawns using a spot assay; (3) isolation of single plaques; (4. BackgroundAll Shigella flexneri serotypes except serotype 6 share a common O-antigen tetrasaccharide backbone and nearly all variations between serotypes are due to glucosyl and/or O-acetyl modifications of the common O unit mediated by glycosyltransferases encoded by serotype-converting bacteriophages.

Several S. flexneri serotype-converting phages including SfV, SfX, Sf6 and SfII have been.O'Brien AD, LaVeck GD. Purification and characterization of a Shigella dysenteriae 1-like toxin produced by Escherichia coli. Infect Immun. May; 40 (2)– [PMC free article] O'Brien AD, LaVeck GD, Griffin DE, Thompson MR.

Characterization of Shigella dysenteriae 1 (Shiga) toxin purified by anti-Shiga toxin affinity chromatography.Further purification and characterization are achieved by density gradients, electron microscopy studies, and genomic analysis. This straightforward methodology can be applied to the detection, enumeration, and isolation of bacteriophages from any bacterial species, using the appropriate host strain, media, and culture conditions.